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Determination ofd-amino acids. I. Hydrolysis of DNP-l-amino acid methyl esters with carboxypeptidase-Y

✍ Scribed by Peter Marfey; Martin Ottesen


Publisher
Springer-Verlag
Year
1984
Weight
379 KB
Volume
49
Category
Article
ISSN
0105-1938

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The effect of added nucleophiles (methanol and 1,4-butanediol) on the steady-state kinetics of a-chymotryptic hydrolysis of a series of -V-acetyl-L-amino acid methyl esters, R-CH(NHCOCH3)C(O)OCH3, has been studied. As a result, the rate and equilibrium consta.nts of the "elementary" steps of the enz