Determination of β-agonists in urine by an enzyme immunoassay based on the use of an anti-salbutamol antiserum

✍ Scribed by Guy Degand; Anne Bernes-Duyckaerts; Philippe Delahaut; Guy Maghuin-Rogister

• Book ID: 102984219
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 612 KB
• Volume: 275
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80298-y

✦ Synopsis

An enzyme mmmnoassay (EIA) method for ,3-agomsts has been developed usmg an antiserum ratsed m rabbits by nnmumza~on agamst 3-0-salbutamol succmate coupled to bovme serum albumm Horse radish perondase coupled to the same salbutamol denvatwe was selected as tracer The dose of salbutamol winch caused 50% bmdmg mhkutlon was 16 8 pg per well and the hnut of detectIon of the EIA dmzctly performed on dduted urme was 0 14 ng ml-' m urme (when the vanabllity of blank values m samples from untreated ammals was taken mto account) Owmg to the large cross reactMt]es of the antibodIes Hnth a number of /3-agomsts, the present assay 1s pronusmg for the smmltaneous determmation of salbutamol, clenbuterol, mabuterol, terbutalme, cunaterol and probably also other &agomsts anth a reti-butyl or isopropyl group at the extremrty of the ahphak side cham of the molecule K&words Enzymatic methods, Immunoassay, &Agomsts, Cattle, Clenbuterol, Urme,