Determination of α-amanitin and β-amanitin in human biological fluids by high-performance liquid chromatography

✍ Scribed by F. Jehl; C. Gallion; P. Birckel; A. Jaeger; F. Flesch; R. Minck

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 607 KB
• Volume: 149
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90474-9

✦ Synopsis

A high-performance liquid chromatographic assay of cu-amanitin and @-amanitin in human serum, urine, or stomach washings is described. Sample preparation involves a chemical step with deproteinization and organic solvent treatment, and a selective cleanup and concentration step on reversed-phase prepacked cartridges. Separations are performed on a reversed-phase analytical column under isocratic conditions with uv detection at 280 nm. The method allows the quantitation of OI-and fl-amanitin separately with a detection limit of 10 rig/ml for both tOXinS.