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Determination of xylose and glucose in a flow-injection system with PQQ-dependent aldose dehydrogenase

✍ Scribed by Maria Smolander; Julia Cooper; Wolfgang Schuhmann; Martin Hämmerle; Hanns-Ludwig Schmidt

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
846 KB
Volume
280
Category
Article
ISSN
0003-2670

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✦ Synopsis

Application of pyrroloquinoline quinone (PQQJ-dependent aldose dehydrogenase (ALDH) for the amperometric determination of xylose and glucose is described. Different flow injection configurations were investigated to optimize the measurement. The highest current densities could be obtained when the enzyme was immobilized directly on a graphite electrode. The best stability was achieved with controlled-pore glass (CPGJ-immobilized ALDH. ALDH-CPG was also successfully used for the measurement of real fermentation samples. The measurement of samples containing only xylose as a carbon source correlated very well with high-pressure liquid chromatographic measurement, the correlation coefficient and conversion factor between the methods being 0.98 and 0.96, respectively. Also samples containing both zylose and glucose could be measured satisfactorily when the measurement with ALDH was combined with separate glucose measurement.

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