Determination of various steroids in normal adrenal glands and adrenocortical tumours by liquid chromatography/atmospheric pressure ionization mass spectrometry

✍ Scribed by Funito Komatsu; Masaaki Morioka; Yukitoshi Fujita; Kazunori Sugahara; Hiroyuki Kodama

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 401 KB
• Volume: 30
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.1190300507

✦ Synopsis

Seven steroids in normal adrenal gland and those of patients with aldosteronoma or Cushing's adenoma were measured by liquid chromatography/mass spectrometry with an atmospheric pressure ionization interface system.

Using this system, quasi-molecular ions (M + HI + of standard cortisol, corticosterone, 17a-hydroxyprogesterone, ll/Y-hydroxyandrostenedione and Atandrostenedione and [(M + H) -H,OJ + ions of aldosterone and dehydroepiandrosterone were observed as their base peaks, and quasi-molecular ions IM + H J + and I(M + H) -H,OJ+ ions of seven steroids were clearly observed in chromatograms of biological samples. The ratio of glucocorticoid (cortisol) to mineralocorticoid (corticosterone) content was measured, and found to be significantly lower for aldosteronomas and higher for Cushing's adenomas than for normal adrenals. These findings indicate that the method had sufficient sensitivity for the identification and simultaneous measurement of a variety of adrenal gland steroids.