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Determination of triacylglycerol lipase activity using carbon-13-labeled triacylglycerols and nuclear magnetic resonance spectroscopy: Evidence that hepatic lipase hydrolyzes medium-chain triacylglycerols

✍ Scribed by R. J. Dimand; E. M. Bradbury; K. L. Cox


Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
270 KB
Volume
9
Category
Article
ISSN
0740-3194

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✦ Synopsis


Triacylglycerol lip= activity was studied using glycerol [ 1-I3C] trioctanoate mixed with postheparin rat plasma. I3C NMR spectroscopy demonstrated triacylglycerol hydrolysis into free fatty acids with no difference at the 1,3 or 2 glycerol positions. There was no inhibition by high sodium concentration, consistent with lipase of hepatic origin.