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Determination of Trace Proteins by Rayleigh Light Scattering Technique with Indophenol Blue

✍ Scribed by Zhong, Hui ;Xu, Jing-Juan ;Chen, Hong-Yuan


Publisher
Springer-Verlag
Year
2004
Weight
164 KB
Volume
148
Category
Article
ISSN
0344-838X

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Based on the enhancement of Rayleigh light scattering (RLS) of acid green 2.5 (Ag25) by proteins, a new quantitative determination method for proteins in aqueous solutions has been developed. This assay is very sensitive (0.136-10.20 ug/ml), rapid (~2 min) and simple (one-step assay and common fluor