Determination of trace levels of transition metal ions in fermentation broth by ion-pair chromatography

✍ Scribed by Gerald Schuster; Werner A. Hampel

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 490 KB
• Volume: 258
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85102-c

✦ Synopsis

A method for the determmatlon of trace levels of transItion metal 1011s such as Fez+, Mn'+ and Zn2+ by hquld chromatography usmg reversed-phase Ion-pair separation, post-columu denva&atlon urlth 4_(2-pyrldylazo)resorcmol and spectrophotometnc detection at 520 and 540 nm IS described Even tugh levels of typlcal fermentation me&a constituents such as sugars and cltnc acid do not have a negatwe effect on the determmatlon The apphcatlon of the method to fig 1-l levels of Fe*+ and Mn*+ eons m atrlc acid fermentatum broth suffers from relatively high osclllatmg rmlse signals and unpredictable retention times, wluch IS caused by the cohmm capacity bemg exceeded at lugh concentrations of catIons such as K+, Mg2+ and Ca'+ and certam orgamc substances Dlgltal filtermg procedures were used to Improve the sIgnal-to-noise ratlo and correct peak ldentlficatlon was ensured by determmmg the absorption ratlo A5&As2,,