Determination of Trace Amounts of Iron(II,III) in Natural Water by Reversed-Phase High-Performance Liquid Chromatography

✍ Scribed by H. Inoue; K. Ito

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 313 KB
• Volume: 49
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1994.1035

✦ Synopsis

Trace amounts of iron(II,III) ions in natural water were determined by reversed-phase high-performance liquid chromatography (HPLC) using precolumn derivatization with 1-(2pyridylazo)-2-naphthol (PAN). A good separation of PAN chelates with iron(II,III), nickel(II), and copper(II) ions was achieved with a chemically bonded ODS column using (\mathrm{THF} / \mathrm{H}_{2} \mathrm{O} /) methanol ((30 / 50 / 20, \mathrm{v} / \mathrm{v} / \mathrm{v})) containing (0.01 \mathrm{M}) tetrabutylammonium thiocyanate as eluent. The calibration graphs of iron(II), iron(III), nickel(II), and copper(II) ions were linear in the concentration range of (0-100 \mathrm{ng} \mathrm{cm}^{-3}). The detection limits of iron(II), iron(III), nickel(II), and copper(II) ions were (6.19,6.38,6.72), and (8.45 \mathrm{ng} \mathrm{cm}^{-3}) with relative standard deviations ((n=10)) of (7.24,6.63,6.76), and (1.44 %) at a concentration level of (10 \mathrm{ng} \mathrm{cm}^{-3}), respectively. It was demonstrated that the proposed HPLC method with PAN chelates is useful for the determination of heavy metal ions in natural water. O 1994 Academic Press, Inc.