Using nuclei isolated from less than 0.2 g tissue or 10' cells, a method is presented for the quantitative determination of amounts of DNA per cell at the picogram level. This technique is based on the enhanced fluorescence of 4',6-diamidino-2-phenylindole (DAPI) when it binds to DNA. A rapid, one-s
β¦ LIBER β¦
Determination of total intracellular lipid content by flow cytofluorometry
β Scribed by V.V. Tertov; A.N. Orekhov; S.A. Rudchenko; D.N. Mukhin; V.N. Smirnov; Yu.G. Molotkovsky; L.D. Bergelson
- Book ID
- 115758708
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 950 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0006-291X
No coin nor oath required. For personal study only.
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