Determination of total captopril in dog plasma by HPLC after prelabelling with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F)

✍ Scribed by Toshimasa Toyo'oka; Kazuhiro Imai; Yukinori Kawahara

• Book ID: 103910621
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 516 KB
• Volume: 2
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(84)80050-3

✦ Synopsis

A HPLC method has been developed for the determination of captopril, an angiotensin-converting enzyme (ACE) inhibitor, in dog plasma. The plasma was deproteinized with trichloroacetic acid (TCA) containing N-(3-mercapto-l-oxopropyl)piperidine-2-carboxylic acid (SQ 25,233) as an internal standard, adjusted to pH 9.5 with borate buffer containing tri-n-butyl phosphine (TBP) to reduce the oxidized captopril, and treated with a thiol specific reagent, SBD-F. The fluorescent derivatives of captopril and the internal standard were separated from other interfering peaks on a reversedphase column and determined by HPLC. The proposed method was simple, selective and sensitive. The total plasma concentration of captopril reached a maximum of 3.04 + 0.54 &ml (mean + S.E.) 60 min after administration of the drug.