Determination of total 3-chloropropane-1,2-diol (3-MCPD) in edible oils by cleavage of MCPD esters with sodium methoxide
✍ Scribed by Rüdiger Weißhaar
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 174 KB
- Volume
- 110
- Category
- Article
- ISSN
- 1438-7697
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✦ Synopsis
Abstract
A method for the determination of total 3‐chloropropane‐1,2‐diol (3‐MCPD) in edible fats and oils was presented. 3‐MCPD was released from 3‐MCPD fatty acid esters by transesterification with NaOCH~3~/methanol. After derivatization with phenylboronic acid, 3‐MCPD was determined by GC‐MS. Deuterium‐labeled 3‐MCPD was used as internal standard. In a model experiment, it was shown that acidic hydrolysis with methanol/sulfuric acid, which is normally used for the release of 3‐MCPD from its esters, can cause problems because under acidic conditions additional 3‐MCPD can be formed. No additional 3‐MCPD was formed using NaOCH~3~/methanol for transesterification. Eleven samples of cold‐pressed and refined safflower oils were analyzed with this method. Levels of total 3‐MCPD were in the range from <100 up to 3200 µg/kg.