Determination of the volumes of void zones in packed chromatographic columns

✍ Scribed by Mohammad N. Rathor; Bryan A. Buffham; Geoffrey Mason

• Book ID: 104145716
• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 699 KB
• Volume: 445
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84506-4

✦ Synopsis

In gas chromatography

there is a pause in flow when sorbable material enters the packing and is absorbed by the stationary phase. There is a complementary surge in flow when the band of sorbed material leaves the packing. When a band passes a void zone, these effects combine to produce a doublet relative to the base-line on a recording of flow-rate against time. Such fluctuations can be measured by using a sensitive differential capillary flow meter. A theory is developed that enables the volumes of void zones in packed chromatographic columns to be found by measuring fluctuations in flow-rate observed when bands of sorbable material pass through the void zones. The theory is confirmed by experiment.

The volume of a 0.06-cm3 void zone was estimated to within about 3%.