The effect of pressure on the unfolding of the native (N) and molten globule (MG) state of canine milk lysozyme (CML) was examined using ultraviolet (UV) spectroscopy at pH 4.5 and 2.0, respectively. It appeared that the thermally induced unfolding was promoted by the increase of pressure from atmos
✦ LIBER ✦
Determination of the Volume Changes for Pressure-Induced Transitions of Apomyoglobin between the Native, Molten Globule, and Unfolded States
✍ Scribed by Vidugiris, Gediminas J.A.; Royer, Catherine A.
- Book ID
- 119411397
- Publisher
- Biophysical Society
- Year
- 1998
- Tongue
- English
- Weight
- 170 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0006-3495
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## Abstract A few studies indirectly support the existence of an intermediate in the transition of Ca^2+^‐saturated bovine α‐lactalbumin (α‐LA) from the native (N) to the acidic (A) state, known as the molten globule state. However, direct experimental evidence for the appearance of this intermedia