Determination of the sulfhydryl and disulfide groups in proteins by methylation, treatment with raney nickel and infrared spectrometry
โ Scribed by M. Krsteva; B.V. Alexiev; C.P. Ivanov; B. Yordanov
- Publisher
- Elsevier Science
- Year
- 1965
- Tongue
- English
- Weight
- 805 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0003-2670
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Two new reagents for the titration of sulfhydryl groups in peptides and proteins and for their temporary blocking with the thiomethyl group have been developed. The sulfhydryl groups in cysteine, glutathione, and papain react quantiatively under mild conditions with these reagents, methyl 3-nitro-2-
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