Determination of the stability of complexes between DNA and the thiazole orange derivatives TO6 and TOTO by surface-enhanced resonance Raman spectroscopy
✍ Scribed by M. Nissum; J. P. Jacobsen; O. Faurskov Nielsen; P. Waage Jensen
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 179 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1075-4261
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✦ Synopsis
Complexes of the two thiazole orange derivatives TO6 [1-( N,N-tetramethyl-1,3-propanediaminopropyl )-4-[3-methyl-2,3-dihydro(benzo-1,3-thiazole )-2methylidene] quinolinium triiodide] and TOTO [1,1 -(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)bis-4-[3-methyl-2,3-dihydro(benzo-1,3-thiazole)-2-methylidene] quinolinium tetraiodide] with DNA oligonucleotide strands are investigated by the use of surface-enhanced resonance Raman spectroscopy. TO6 and TOTO contain protons that are exchangeable with deuterium when dissolved in D 2 O. The exchange sites can be identified by use of nuclear magnetic resonance spectroscopy. The degree of exchange observed in the surface-enhanced resonance Raman spectra is used to measure the stability of the complexes formed. TOTO forms a highly stable complex with the d(5 -CCGCTAGCG-3 ) : d(5-CGCTAGCGG-3 ) oligonucleotide, whereas a less stable complex is formed with d(5 -CGCGTTAACGCG-3 ) 2 , indicating some degree of site specificity in the binding of TOTO to DNA. TO6 does not bind strongly to any single site in the d(5-CGCGTTAACGCG-3 ) 2 oligonucleotide.