Determination of the position of the O-acetyl group in a β-(1 → 4)-mannan (acemannan) from Aloe barbardensis Miller

✍ Scribed by Sukumar Manna; Bill H. McAnalley

• Book ID: 102991484
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 220 KB
• Volume: 241
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(93)80122-u

✦ Synopsis

Acemannan, a p-(1 + 4)-linked acetylated mannan' (mannose content, 97%) isolated from the leaves of Aloe barbardensis Miller, was subjected to methylation analysis according to Arnarp et aL2 in order to determine the position of the 0-acetyl groups3. Acemannan was treated with methyl trifluoromethanesulfonate in trimethyl phosphate in the presence of 2,6-di-tert-butylpyridine2'4.

The resultant partially methylated acemannan was subjected to alditol acetate formation5 and subsequently analyzed by GLC-MSG. The peak at 10.61 min in the GLC-MS shows major fragments at m/z 43, 102,