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Determination of the pattern of labelling of carbon atoms 1, 2, 3 and 6 of D-[U-14C] glucose by chemical methods

✍ Scribed by J. C. Turner

Publisher: John Wiley and Sons
Year: 1967
Tongue: French
Weight: 783 KB
Volume: 3
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.2590030306

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✦ Synopsis

Uniformly labelled D- [Wc] glucose obtained from Canna Indica and from Chlorella was converted into several derivatives and degraded by chemical methods to yield products that separately contained carbon atoms I , 2, 3 and 6. The specijic activities of the various compounds were determined by liquid Jcintillation counting and from the results the pattern of labelling in carbon atoms I , 2, 3 and 6 of the two glucose samples was obtained. The labelling was found to be uniform within the limit3 of experimental error.

EXPERIMENTAL

Uniformly labelled D-[14C] glucose prepared from leaves of Canna Indica (Product Code Number CFB.2), uniformly labelled D-[14C] glucose prepared from cell walls of Chlorella (Product Code Number CFB.35) and D-[l-l4C] glucose (Product Code Number CFA.204), were all from the Radiochemical Centre, Amersham, England. The radiochemical purities of these compounds were as follows : CFB.2, by dilution analysis, 99 %; by paper chromatography in two solvent systems 99 and 99 %. CFB.35, by dilution analysis, 98 %; by paper chromatography

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