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Determination of the N-terminal Amino-acid in Polypeptides and Proteins

✍ Scribed by SIGNOR, ANGELO; PREVIERO, ALDO; TERBOJEVICH, MARIA


Book ID
109641495
Publisher
Nature Publishing Group
Year
1965
Tongue
English
Weight
249 KB
Volume
205
Category
Article
ISSN
0028-0836

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Proteins or peptides having an \(\mathrm{N}\)-terminal-blocked amino acid were successively digested by pronase \(E\), proteinase \(K\), and carboxypeptidase \(Y\). The \(\mathbf{N}\)-blocked amino acids released from proteins or peptides were derivatized with 9 -anthryldiazomethane (ADAM) to the co