Microidentification of N-Terminal-Blocke
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Y. Kawakami; S. Ohmori
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Article
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1994
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Elsevier Science
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English
β 540 KB
Proteins or peptides having an \(\mathrm{N}\)-terminal-blocked amino acid were successively digested by pronase \(E\), proteinase \(K\), and carboxypeptidase \(Y\). The \(\mathbf{N}\)-blocked amino acids released from proteins or peptides were derivatized with 9 -anthryldiazomethane (ADAM) to the co