We have identified gene products corresponding to hlyC, hlyA and hlyD encoded by the Escherichia coli haemolytic determinant 2001 of human origin cloned into the recombinant plasmid pLG570. The product of hlyC is required for the "activation" of the inactive 107K polypeptide encoded by the hlyA gene
Determination of the molecular weight of Escherichia coliα-haemolysin
✍ Scribed by Peter H. Williams
- Book ID
- 109310217
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 298 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0378-1097
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The haemolysin (hly) determinant of the plasmid pHly152 contains an IS2 element at 469 bp upstream of the hlyC gene. The sequence at the other (right-hand) end (RS) also shows multiple hybridization with the plasmid pHly152 and the chromosome of some Escherichia coli strains but the nucleotide seque
The synthesis and secretion of the toxic exoprotein e-haemolysin of E. coli PM152 is coded by the transmissible plasmid pHly152 (41 x 10 6 dalton) as shown by the transformation of the plasmid DNA and the isolation of mutants that are specifically altered in the synthesis and transport of haemolysin