Determination of the Ks values during the growth of Alcaligenes eutrophus on phenol, 2,4-dichlorophenoxyacetic acid and fructose
✍ Scribed by R. H. Müller; W. Babel
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 367 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0138-4988
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✦ Synopsis
The determination of the Ksvalues presented here is based on the estimation of the stationary substrate concentrations in continuous cultivation experiments. The separation of biomass from the suspension was performed by an ultrafiitration step which succeeded within one second. The decay of substrate concentration during sampling was calculated to amount to less than 6% of the stationary substrate concentration at relevant growth rates. The KS values derived from these reduced substrate concentrations deviated by only 10% from the theoretical values at a biomass concentration of about 1 g /l. Thus relevant kinetic parameters can be calculated from the data obtained by this procedure. Values of 11.59 and 14 pM were obtained with 2.4-dichlorophenoxyacetic acid (2.4-D), phenol and fructose, respectively. Similar KS values were derived with 2.4-D and fructose by using a respirationbased determination for reasons of comparison. With phenoI this value was only 7 pM which is ascribed to a physiological background.