✦ LIBER ✦
Determination of the isotope distribution in malate-14C with fumarase-negative lactic acid bacteria
✍ Scribed by J. Winter; O. Kandler
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 342 KB
- Volume
- 112
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
No fumarase activity could be found in whole cells or in cell-free crude extracts from Leuconostoc mesenteroides or Lactobacillus curvatus. The degradation of L-malate-4-14C by these organisms yielded more than 95% of the label as 14CO2. It is therefore recommended that these organisms, rather than Lactobacillus plantarum, should be used in the determination of isotope distribution in L-malate-14C, since L. plantarum exhibits a significant fumarase activity and thus randomizes malate prior to the decarboxylation of this substance by the malolactic enzyme.