Determination of the glycosidic torsion angles in uniformly13C-labeled nucleic acids from vicinal coupling constants3JC2/4−H1′and3JC6/8−H1′

The 2D {31P} Spin-Echo-Difference Consta

The 2D {31P} Spin-Echo-Difference Constant-Time [13C, 1H]-HMQC Experiment for Simultaneous Determination of 3JH3′P and 3JC4′P in 13C-Labeled Nucleic Acids and Their Protein Complexes

✍ Thomas Szyperski; César Fernández; Akira Ono; Kurt Wüthrich; Masatsune Kainosho 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 163 KB

A two-dimensional { 31 P} spin-echo-difference constant-time [ 13 C, 1 H]-HMQC experiment (2D { 31 P}-sedct-[ 13 C, 1 H]-HMQC) is introduced for measurements of 3 J C4P and 3 J H3P scalar couplings in large 13 C-labeled nucleic acids and in DNA-protein complexes. This experiment makes use of the fac