Determination of the formation constant of ATP—cobalt(II) complex(ATP—Co)2− by A′pH stat′ titration method
✍ Scribed by J. Galea; G. Ferroni; J.P. Belaich
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 315 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0013-4686
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✦ Synopsis
Ah&-A
technique of pH titration is used for the study of the formation of the ATP Co'complex at diverse temperatures. The stability constank?, as also the corresponding thermodynamic quantities, obtained for an ionic strength 0.2 in tetramethylammonium bromide are reported and compared with these of ATP Mn'-and ATP Mg'-complexes. NOMENCLATURE A = total nucleotide concentration. A = ~~~+~-] + [ATPH3-1, before addition of A = [ATF-1 + [ATPHJ-] + [ATPHCo-] + CATPCo'-1, after addition of Colt. b = analytic concentration of Co'+ corresponding to the first addition of Co3+. c = analytic concentration of Co'+ after the second addition of Co'+, (c = 2b). x,y = respectively concentration of ATP-Co'-, at equilibrium after the first (b) and the second (c) addition of co*+. (I; with i = 0.1 or 2 = concentration of ATP*-resoectivelv before the addition of Co'+, after the firs; (b) a&l the second (c) additions of Co*+. a: with i = 0.1 0; 2; the same convention for ATPH3as above for ATP4-. Z1 and Z2 = respectively number of equivalents of H+ released after the first (b) and the second (c) additions of co= +. T1 and @ number of equivalents of H* per 1. corresponding respectively to the quantities 2, and Z2_ T1 = 2,/V and r' = ZJV (V = total volume). A. Belaich for her help in microcalorimetry, to Dr. M.