Determination of the epitope specificity of monoclonal antibodies against the inner core region of bacterial lipopolysaccharides by use of 3-deoxy-d-manno-octulosonate-containing synthetic antigens
✍ Scribed by Antoni Rozalski; Lore Brade; Hella-Monika Kuhn; Helmut Brade; Paul Kosma; Ben J. Appelmelk; Shoichi Kusumoto; Hans Paulsen
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 939 KB
- Volume
- 193
- Category
- Article
- ISSN
- 0008-6215
No coin nor oath required. For personal study only.
✦ Synopsis
Partial structures of enterobacterial lipopolysaccharides (LPS) of the Rechemotype, consisting of lipid A and 3-deoxy-D-manno-2-octulosonic acid (Kdo), as well as oligosaccharides and derivatives of Kdo were synthesized and used to characterize the epitope specificity of monoclonal antibodies against Re-mutant LPS. High-molecular-weight antigens, obtained after copolymerization of the respective ally1 glycosides with acrylamide, and the haptenic oligosaccharides were used in immunoprecipitation, immune hemolysis, and in inhibition assays. A monoclonal antibody (clone 20, IgM) recognizing a terminal Kdop group was shown to require for its binding the a-anomeric configuration and OH-4 and OH-5 groups, whereas the C-7-C-8 chain was of minor importance. Another monoclonal antibody (clone 25, IgG,), which recognizes a (2+4)-linked Kdo disaccharide, was