𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of the enantiomeric purity of (−−) 2-(N-propyl-n-2-thienylethylamino)-5-hydroxytetralin (n-0923) by chiral stationary phase HPLC

✍ Scribed by Dirk T. Witte; Jan-Piet Franke; Pieter J. Swart; Rokus A. De Zeeuw

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
250 KB
Volume
4
Category
Article
ISSN
0899-0042

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The determination of the enantiomeric impurity, i.e., the percentage of (+) N−0437 (= N−0924) in several batches of (−−) N‐0437 (= N‐0923) by chiral HPLC is described. Enantiomeric impurities were calculated based on the peak areas of the two baseline separated enantiomers in the chromatogram. The enantiomeric impurities found in different batches ranged from 0.02% to 0.11%. Calibration curves of the two isomers of N‐0437 (Fig. 1,) were made twice to study the reproducibility and linearity of the method. The absorbance ratio, N‐0923/N‐0924, was found to be 1.02 with a relative standard deviation (RSD) of 9% over the whole concentration range used for the calibration curves.

📜 SIMILAR VOLUMES

N-(n-Butylamide) of (S)-2-(phenylcarbamo
N-(n-Butylamide) of (S)-2-(phenylcarbamoyloxy)propionic acid: A new chiral solvating agent, derived from L-lactic acid, for the enantiomeric purity determination of derivatized amino acids
✍ Dario Pini; Gloria Uccello-Barretta; Carlo Rosini; Piero Salvadori 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 252 KB

The N-(n-butylamide) of (S)-2-(phenylcarbamoyloxy)propionic acid, easily prepared starting from the inexpensive L-ethyl lactate, can be used as convenient chiral solvating agent (CSA) to determine the enantiomeric composition of N-(3,5-dinitrobenzoyl)amino acid methyl esters.