Determination of the double bond position in functionalized monoenes by chemical ionization ion-trap mass spectrometry using acetonitrile as a reagent gas
✍ Scribed by Neil J. Oldham; Aleš Svatoš
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 90 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0951-4198
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✦ Synopsis
Acetonitrile may be used as a chemical ionization reagent gas in ion-trap mass spectrometry for the location of double bonds in functionalized long-chain monoenes. [C 3 H 4 N] , derived from acetonitrile, reacts with carbon-carbon double bonds, generating one or a pair of fragment ions characteristic of the position of the double bond in the chain. Tolerance of a range of functional groups is achieved by isolating [C 3 H 4 N] from the other acetonitrile ions before reaction with the alkene. Under these conditions, competing processes such as protonation are minimized, and a different mode of fragmentation from that previously observed with unfunctionalized alkenes 11 is obtained. The technique is highly sensitive (`0.5 ng), allowing its use in the identification of insect pheromones.