Determination of the Dihydropyridine Aryloxypropanolamine Z6568 and its Acidic Metabolites in Plasma, Urine and Tissues by Solid Phase Extraction and Liquid Chromatography/Negative-ion Mass Spectrometry

✍ Scribed by Gregori, Mario; Castelnovo, Pierfrancesco

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 728 KB
• Volume: 31
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(199609)31:9<994::aid-jms365>3.0.co;2-g

✦ Synopsis

A sensitive and selective liquid chromatographic/mass spectrometric assay for the determination of the dihydropyridine aryloxypropanolamine compound 26568 and its monoacidic metabolites in plasma, urine and tissues has been developed and validated. 26568 and the acidic metabolites were isolated from the biological matrices by sotid-phase extraction, separated by reversed-phase high-performance liquid chromatography and detected by mass spectrometry using a thermospray interface. Filament ionization and negative-ion detection were used, monitoring the molecular ion of 26568 and a major fragment of the acidic metabolites. The analysis of spiked plasma, urine and tissues demonstrated good accuracy and precision of the assay with a detection limit of 0.5 ng ml-' for 26568 and 2 ng ml-' for the metabolites. Data relative to the optimization of the extraction procedure, mass spectrometric conditions and the validation protocol are reported. The method was used to investigate the metabolic fate and tissue distribution in laboratory animals.