Determination of the density of theoretical plates and the mass effect in countercurrent electrophoresis

✍ Scribed by H.D Freyer

• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 726 KB
• Volume: 91
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97967-1

✦ Synopsis

The density of theoretical plates, n(cm-l)

= v~/D~rf, in a countercurrent electrophoretic column is given by the effective diffusion coefficient, Dcrf, of the ions and the flow-rate, VL, of the countercurrent solution. Both values and the dependence on each other have been measured.

The mass effect, 1 + .E, based on the differences in ionic mobilities, was determined for the isotope mixture 2ZNa-Z3Na in 1 N acetic acid as the countercurrent solution as a function of the ionic concentration.

It was found that experimentally determined separation factors, Q = (1 + + E)~*S, obtained in the stationary state of the process, are in good agreement with those, calculated from the measured values of n and 1 + E.