๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Determination of the concentration and specific activity of acetone in biological fluids

โœ Scribed by Victor C. Gavino; Bernard Vinet; France David; Michel Garneau; Henri Brunengraber

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
480 KB
Volume
152
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

The concentration of acetone dissolved in liver perfusion medium was determined by injection of the sample into a gas chromatograph equipped with a Carbopack/Carbowax-packed glass column. Interference from labile acetoacetate which readily decomposes to acetone was eliminated by treating the samples with NaBH4 prior to the analysis. Acetone was detected and quantified as 2-propanol. Separation of labeled 2-propanol in the sample by high-performance liquid chromatography allowed the determination of its specific activity. These methods make possible the convenient and rapid determination of acetone concentration and specific activity in biological SmlpleS.

๐Ÿ“œ SIMILAR VOLUMES

The determination of aminoketones in bio
The determination of aminoketones in biological fluids
โœ H.S. Marver; D.P. Tschudy; M.G. Perlroth; A. Collins; G. Hunter Jr. ๐Ÿ“‚ Article ๐Ÿ“… 1966 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 487 KB

The first step in the biosynthesis of porphyrins involves the condensation of glycine and succinyl coeneyme A to form b-aminolevulinic acid (ALA) (1, 2). The enzyme which catalyzes this reaction (ALA synthetase) is of particular interest, since it is an inducible enzyme in mammalian liver (3-6) subj

GLC determination of theophylline in bio
GLC determination of theophylline in biological fluids
โœ Vinod P. Shah; S. Riegelman ๐Ÿ“‚ Article ๐Ÿ“… 1974 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 321 KB

ping m, methylenes and methines), 3.59 (s, 3H, vinyl methoxy), 3.64 and 3.66 (as, 3H total, carbomethoxy), 3.72 (d, lH, J = about 10 Hz, benzylic proton on C-41, 5.00 (s, lH, vinyl), 5.82 (s and an indistinct overlapping signal, 2H plus lH, methylenedioxy and proton on C-3), and 6. 64 and 6.67 (2s,