Determination of the calcium entry blocker verapamil in plasma by capillary gas chromatography with on-column injection

✍ Scribed by Rosseel, M. T. ;Belpaire, F. M.

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 303 KB
• Volume: 11
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240110126

✦ Synopsis

A specific, sensitive, and accurate assay for quantitation of verapamil has been developed. The method involves a single extraction step with n-heptane, followed by evaporation at room temperature under nitrogen. 0.1 pi of the extract was injected into a capillary column coated with crosslinked 5% phenylmethylsilicone. The column separated verapamil, norverapamil, and its internal standard within 15 minutes using temperature programming from 90 to 29OoC. The lower limit of detection was 1 ng/ml forverapamil. Thecalibrationcurvewaslinear in theconcentration range (5-300 ng/ml). Plasma concentration data from dogs receiving intravenous verapamil infusion are presented.