Determination of the apparent transverse and axial dispersion coefficients in a chromatographic column by pulsed field gradient nuclear magnetic resonance

Determination of the Binding Constant of

Determination of the Binding Constant of a Protein Kinase C Substrate, NG(28–43), to Sodium Dodecyl Sulfate via the Diffusion Coefficient Measured by Pulsed Field Gradient Nuclear Magnetic Resonance

✍ Wei-Jyun Chien; Shu-Fang Cheng; Ding-Kwo Chang 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 81 KB

The binding affinity of a protein kinase C substrate, neurogranin peptide NG (28 -43) , to a sodium dodecyl sulfate micelle was analyzed quantitatively by the diffusion coefficient (D s ) of the peptide determined by pulsed field gradient NMR. By use of a two-state model, the fraction of the peptide