Determination of the alcohol denaturants denatonium benzoate (bitrex) and diethyl phthalate by direct flow injection APCI-MS analysis
✍ Scribed by Alvarez-Piñeiro, Ma Elena ;De Alda-Villaizán, Ma Joséa López ;Paseiro-Losada, Perfecto ;Lage-Yusty, Ma Asunción
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 392 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
The recently developed tandem technique, mass spectroscopy with atmospheric‐pressure chemical ionization (APCI‐MS) coupled with direct flow injection, was applied to the determination of Bitrex (denatonium benzoate) and diethyl phthalate in ethanol. The compounds were indentified from their total‐ion chromatograms(m/z 50‐500) and, simultancously, quantified using selected‐ion chromatograms recorded at m/z 223 for diethyl phthalate and m/z 325 for Bitrex. Operating conditions were optimized for soft ionization (positive ion‐mode) with fragmentation limited to that necessary for analyte identification, which was by the external‐standard method. Calibration curves were rectilinear in the concentration rages 2‐30 μg ml^−1^ (Bitrex) and 0.075‐1.2% v/v (diethyl phthalate); measurement precision (RSDs were 11.48% for Bitrex and 2.22% for diethyl phthalate), and detection limits (0.017 μg ml^−1^ and 0.010% v/v, respectively) were adequate for simiultaneous quantitation and with high sensitivity. The lack of any sample pretreatment and the use of flow‐injection analysis meant that the procedure was moere straightforward and rapid than previously reported methods.