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Determination of the active sites serine of the poly (3-hydroxybutyrate) depolymerases of Pseudomonas lemoignei (PhaZ5) and of Alcaligenes faecalis

✍ Scribed by Takeyuki Shinohe; Masaki Nojiri; Terumi Saito; Thomas Stanislawski; Dieter Jendrossek


Book ID
116131982
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
654 KB
Volume
141
Category
Article
ISSN
0378-1097

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✍ Adalgisa Batista; María A. Aponte; Emilio Díaz; Héctor R. Reyes 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 94 KB 👁 2 views

PHB-depolymerase degraded poly[(3-hydroxybutyric acid)-co-(3-hydroxyvaleric acid)] (PHB/V) significantly. No other enzyme used in this study showed activity toward PHB/V. Weight loss and gel permeation chromatography (GPC) studies suggested that enzymatic degradation takes place by a surface erosion