✦ LIBER ✦

Determination of the 13C-Labeling Pattern of Glutamate by Gas Chromatography-Mass Spectrometry

✍ Scribed by M. Beylot; F. David; H. Brunengraber

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 360 KB
Volume: 212
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1364

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✦ Synopsis

We present a simple technique for determining the 13C-labeling pattern of glutamate by gas chromatography-mass spectrometry. Glutamate is derivatized with dimethylformamide dimethyl acetal (Methyl-8R). The dimethylaminomethylene methyl ester derivative of glutamate yields fragment ions that allow calculation of 13C enrichment on each carbon. The technique was tested by analyzing glutamate from rat livers perfused with various 13C tracers. The labeling patterns obtained agreed with theoretical calculations or patterns reported with 14C and 13C tracers.

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