Determination of taxane concentrations in Taxus canadensis clippings using high performance liquid chromatographic analysis with an internal standard
✍ Scribed by Brian J. Cass; Donald S. Scott; Raymond L. Legge
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 67 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0958-0344
No coin nor oath required. For personal study only.
✦ Synopsis
Clippings of Taxus canadensis were collected and divided into needles, twigs and whole clippings samples. The biomass was extracted and analysed for taxanes using high performance liquid chromatography. The addition of n-octylbenzamide as an internal standard was found to reduce the standard deviation of calculated yields by a third. Paclitaxel was found to be highest in needles (0.0434% dry wt). The presence of paclitaxel was confirmed by photodiode array spectra. Impurities were found to co-elute with other taxanes. A compound was tentatively identified as 9-dihydro-13-acetylbaccatin III based on residence time and UV spectra. The effect of storage on paclitaxel content was investigated and it was found that paclitaxel was stable in dried needles stored at room temperature for at least 1 year.