Determination of taurocholic acid in bile salts**Presented at the Twenty-Second Annual Meeting of the American Drug Manufacturers' Association held at Hot Springs, Virginia, May 8-11, 1933.
✍ Scribed by Willson, F.E.
- Publisher
- Elsevier
- Year
- 1933
- Weight
- 325 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0898-140X
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✦ Synopsis
We have made it a rule not to use for the test any milk that exceeded an acidity of 0.15 per cent calculated as lactic acid and we have abandoned altogether any idea of adjusting milk that is more acid by adding sufficient alkali. If the milk is not naturally a t the proper acidity, we do not use it.
"I am decidedly of the opinion that in the next revision of the National Formulary adjustment of the milk by the addition of alkali should be omitted but that preferably a test for acidity should be included and milk that runs more than 0.15 per cent acidity as lactic acid should not be used.
"Of course, with a reference standard for direct comparison it does not make so much difference if the acidity varies somewhat and the adoption of such a standard would help to eliminatc the errors that may occur due to variations in sources of supply of milk." Laboratory N6. 4 states, "These tests indicate that, compared with the laboratory test last year, this rennin standard has decreased slightly in activity." Laboratory No. 5 concludes that the standard rennin "curdles the milk in slightly less time than we reported last year." This laboratory also makes the suggestion that the titration for acidity can be made more sensitive, if five drops of the milk being titrated be added to 5 cc. of water. The pink color shows up better. It is customary to use 50 cc. of the milk, to which 0.5 cc. of phenolphthalein solution has been added. After the preliminary titration a second one should be made using just P little less than expected amount of alkali.
Laboratory No. 6 remarks that this year's results "are very close to those which we reported last year." This year's work confirms the previous conclusion that the National Formulary Fifth Edition Method is unsatisfactory and unreliable, and that a standard rennin would overcome the difficulties inherent in the present method. The work adds another year's cumulative evidence for the exceIlent keeping quality of the A. D. M. A. reference rennin and its suitability as a standard in case a method based upon the use of a standard is adopted in the National Formulary Sixth Edition.
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