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Determination of Stevioside in Plant Material and Fruit Teas

✍ Scribed by Tomáš Vaněk; Aleš Nepovı́m; Pavel Valı́ček

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
216 KB
Volume
14
Category
Article
ISSN
0889-1575

No coin nor oath required. For personal study only.

✦ Synopsis

Stevioside, a diterpene glucoside from Stevia rebaudiana, belongs to the strongest natural sweeteners. Its potential widespread use requires an easy and accurate analytical method. A simple method for stevioside analysis based on the water extraction, hydrophobic chromatography (Sep-Pak C cartridges) and HPLC using linear gradient of acetonitrile in water is described. The feasibility of this procedure was tested analyzing the stevioside level in Stevia leaves as well as in tea &&Fruit tea with Stevia''. Stevioside content did not show statistically signi"cant di!erence when a set of 20 tea bags randomly chosen from "ve boxes was determined (P(0.01). The mean value of stevioside content in fruit tea with Stevia was 8.14$2.71 mg/mL. The described method proved to be fast and friendly to the environment by minimization of organic solvent consumption.

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