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Determination of steroidal saponins in Tribulus terrestris by reversed-phase high-performance liquid chromatography and evaporative light scattering detection

โœ Scribed by M. Ganzera; E. Bedir; I.A. Khan

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
216 KB
Volume
90
Category
Article
ISSN
0022-3549

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โœฆ Synopsis

This paper describes the ยฎrst analytical method suitable for the determination of steroidal saponins in Tribulus terrestris. A separation by high performance liquid chromatography (HPLC) was achieved by using a reversed-phase (RP-18) column, evaporative light scattering (ELS) detection, and a water/acetonitrile gradient as the mobile phase. The marker compound, protodioscin, was detected at a concentration as low as 10.0 mg/mL. Several different samples of plant material were successfully analyzed, and depending on origin and plant part used for extraction, signiยฎcant differences in the composition of the saponins were observed. The analysis of market products showed considerable variations of 0.17 to 6.49% in the protodioscin content.

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