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Determination of sphingosine kinase 2 activity using fluorescent sphingosine by capillary electrophoresis

✍ Scribed by Philip M. Yangyuoru; Antony C. Otieno; Simon M. Mwongela

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
464 KB
Volume
32
Category
Article
ISSN
0173-0835

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## Abstract Sphingosine kinase (SphK) is a key enzyme in modulating the levels of sphingosine 1‐phosphate (S1P) as well as an important enzyme in numerous biological responses. Using C17‐sphingosine as a substrate, we established a rapid, sensitive and highly efficient method for determination of S