Determination of serum atenolol using HPLC with fluorescence detection following isolation with activated charcoal

✍ Scribed by T. Alebić-Kolbah; F. Plavšić; A. Wolf-Čoporda

• Book ID: 103911236
• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 274 KB
• Volume: 7
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(89)80193-1

✦ Synopsis

In order to determine the bio-availability and pharmacokinetics of two oral 100 mg atenolol preparations, a simple analytical method was developed. Atenolol was determined in serum submitted to an extraction procedure consisting of: (a) adsorption of atenolol to activated charcoal at pH 11, (b) washing the charcoal with water to remove co-extracts and (c) elution of atenolol from the charcoal with organic solvent. The extracts were then analysed by high-performance liquid chromatography (HPLC) with fluorescence detection. The pharmacokinetic parameters obtained from eight healthy humans involved in a clinical bioavailability trial are also presented.