Determination of pyruvate, acetoacetate, lactate, and 3-hydroxybutyrate in plasma of patients with diabetes mellitus by capillary isotachophoresis
✍ Scribed by Ludmila Kr̆ivánková; Petr Boc̆ek
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 305 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1040-7685
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✦ Synopsis
Abstract
An isotachophoretic method was developed and optimized for simultaneous determination of pyruvate, acetoacetate, lactate, and 3‐hydroxybutyrate in 1–10 μL of untreated heparin plasma of patients with diabetes mellitus. A commercial instrument with a column switching system was used. The preseparation capillary was filled with the leading electrolyte of pH 4.2 and the analytical capillary was filled with leading electrolyte of pH 3.0. The concentrations of individual ketone bodies and lactate found in human plasma varied within 0.01–1.4 mmol/L and 0.8–11.8 mmol/L, respectively. Analysis time was about 30 min.