Determination of pseudo-α- and pseudo-β-dl-glucose by gas-liquid chromatography, high-performance liquid chromatography, and enzymatic colorimetry with glucose 2-oxidase

✍ Scribed by Jun Okuda; Tadao Taguchi; Tetsuo Suami; Seiichiro Ogawa

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 504 KB
• Volume: 162
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90407-6

✦ Synopsis

Three methods have been developed for measuring pseudo-a-and pseudo-&DL-glucose (pseudo-j3-&glucose), synthetic compounds in which the ring oxygens of a-and &DL-&cos~ (&~-glucose) have been replaced by a methylene group. Moderate sensitivity in the determination of these pseudo-glucoses dissolved in human serum was obtained by GLC (0.1 nmol) and HPLC (0.5 nmol). The calorimetric determination with glucose 2-ox&se, peroxidase, and 2,2'-axino-di-(3-ethyibenzothiazoline-6-stdfonic acid) was satisfactory for the assay of pseudoaand pseudo-&DL-ghtcose (respective sensitivities: 25 and 5 nmol). The addition of hexokinase to the calorimetric assay system made it possible to eliminate glucose present in the sample, such as serum, and the remaining pseudo-a-or pseudo-&DL-glucose in the sample solution could then'be measured by a calorimetric method using glucose 2-oxidase. The methods described can be used for biochemical studies involving pseudo-a-and pseudo-&DL-ghCoSe.