Determination of Protein Backbone 13 CO Chemical Shift Anisotropy Tensors in Solution
β Scribed by Pang, Yuxi; Zuiderweg, Erik R. P.
- Book ID
- 126790852
- Publisher
- American Chemical Society
- Year
- 2000
- Tongue
- English
- Weight
- 103 KB
- Volume
- 122
- Category
- Article
- ISSN
- 0002-7863
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π SIMILAR VOLUMES
## Abstract The principal components and orientations of the chemical shift anisotropy (CSA) tensors of nearly all ^13^C carbonyl nuclei in a small protein have been determined in isotropic solution by a combination of three complementary crossβcorrelation measurements.
A solid state NMR technique for the determination of peptide backbone conformations at specific sites in unoriented samples under magic angle spinning (MAS) is described and demonstrated on a doubly labeled polycrystalline sample of the tripeptide AlaGlyGly and a sextuply labeled lyophilized sample