Determination of Propranolol by Capillary Electrophoresis with End-Column Amperometric Detection

✍ Scribed by Xiaoxia Bai; Tianyan You; Hanwen Sun; Xiurong Yang; Erkang Wang

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 125 KB
• Volume: 12
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/(sici)1521-4109(200005)12:7<535::aid-elan535>3.0.co;2-y

✦ Synopsis

A capillary electrophoresis-amperometric detection system was developed for the determination of propranolol (PRO) at a 33 mm carbon ®ber microdisk electrode (CFE). The cyclic voltammogram, the hydrodynamic voltammograms and the effect of pH were studied. Under the optimum conditions: separation voltage 15 kV; injection 3 s at 15 kV; 10 mM pH 7.5 phosphate buffer, 1.15 V (vs. Ag=AgCl) detection potential, the detection limit (LOD) for PRO was 0.05 mM (S=N 3). The response for PRO was linear over two orders of magnitude with a linear correlation coef®cient of 0.994. The feasibility of this method was demonstrated by the detection of PRO in urine sample.