Determination of polyhydroxy antibiotics by capillary zone electrophoresis with amperometric detection at a nickel electrode

✍ Scribed by Xiaoming Fang; Jiannong Ye; Yuzhi Fang

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 530 KB
• Volume: 329
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(96)00092-x

✦ Synopsis

Capillary zone electrophoresis

(CZE) with amperometric detection at a nickel electrode for the determination of polyhydroxy antibiotics in alkaline solution is described. The electrode used was a 300 urn diameter Ni disk electrode operated in a wall-jet configuration. With a working potential of +0.54 V and an 80 cm capillary (o.d. 360 urn, i.d. 25 urn) at 15 kV in the electrophoretic medium of O.lOmol/l NaOH, a sample mixture containing seven different polyhydroxy antibiotics was separated within 20min. The linearity varied over three orders of magnitude of concentration, the detection limit was 0.50 umol/l, for doublemycin, and the relative standard deviation of the peak current was about 5.5% in a period of 8 h. The method has been successfully employed to the determination of the purity of kanamycin, and the content of amikacin in human urines.