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Determination of plasmid copy number and stability in Clostridium acetobutylicum ATCC 824

โœ Scribed by Sang Yup Lee; Lee D. Mermelstein; Eleftherios Terry Papoutsakis


Book ID
109319345
Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
352 KB
Volume
108
Category
Article
ISSN
0378-1097

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The genes coding for enzymes involved in butanol or butyrate formation were subcloned into a novel Escherichia coli-Clostridium acetobutylicum shuttle vector constructed from pIMP1 and a chloramphenicol acetyl transferase gene. The resulting replicative plasmids, referred to as pTHAAD (aldehyde/alco