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Determination of plasmalogen contents of phospholipid classes by reaction micro-thin-layer chromatography

โœ Scribed by V.E. Vaskovsky; V.M. Dembitzky

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
239 KB
Volume
115
Category
Article
ISSN
1873-3778

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โœฆ Synopsis

Determination of piasmalogept contents of phospholipid classes by reaction micro-thin-layer chramatagraptry

Plasmal~gens a~ widespread in nature and appear to be essential in some bio&i&zf processes -_ ' =. Different methods for their analysis have been described4s5

and new ones have recently been suggested 6. Reaction thin-layer chrom2togFaphy (TIC) is the most convenient procedure far the determination of pIasmalogen contents of separated Iipids7-g. We hav e combined reaction TLC principles with our micro-TLC technique of Iipid anaIysisLO.zL and obtained a simple and sensitive method for plasmalogen quantitatian in phospholipid classes.

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Microparticles in the circulation activate the coagulation system and may activate the complement system via C-reactive protein upon conversion of membrane phospholipids by phospholipases. We developed a sensitive and reproducible method to determine the phospholipid composition of microparticles. S