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Determination of plasma pyridoxal 5′-phosphate by an enzymatic-high-performance liquid chromatographic procedure

✍ Scribed by Peter H. Yu

Book ID
107714712
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
414 KB
Volume
181
Category
Article
ISSN
0003-2697

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Measurement of plasma pyridoxal 5′-phosp
Measurement of plasma pyridoxal 5′-phosphate by combination of an enzymatic assay with high-performance liquid chromatography/electrochemistry
✍ Bruno Lequeu; Jean-Claude Guilland; Jacques Klepping 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 375 KB

An assay method for the determination of pyridoxal 5'-phosphate (PLP) in plasma is presented. The procedure is rapid and requires only a small volume. The method includes PLP-dependent enzymatic decarboxylation of L-tyrosine to tyramine (enzyme used%tyrosine apodecarboxylase (EC 4.1.1.25) from Strep