Determination of plasma levels of exemestane (FCE 24304), a new irreversible aromatase inhibitor, using liquid chromatography/thermospray mass spectrometry

✍ Scribed by C. Allievi; P. Zugnoni; M. Strolin Benedetti; P. Dostert

• Book ID: 102379677
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 437 KB
• Volume: 30
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.1190300506

✦ Synopsis

A high performaoce liquid chromatographic mass spectrometric method with thermospray ionization for the determination of concentratioas of exemestane (dmethylena~drosta-1,4-diene-3,17dione, FCE 24304) in human plasma has been developed. The sensitivity of the method allowed the determination of exemestane concentrations as low as 1 ng d-'. A suitable internal standard was used for quantification. The intraday precision (relative standard deviation, RS.D.) ranged from 16.3% near to the lower limit of quaatilkation to 0.3% close to the upper end of the calibration graph. The mean accuracy of the method was found to be 97.8% (RS.D. = 7%). Unlike a previously developed gas chromatographic/mass spectrometric method, the new method proved to be suitable for the determination of unchanged drug plasma levels in exemestane-treated subjects.